Alteration of circulating endothelial cells from acute promyelocytic leuke-mia patients before and after treatment and its influential factors
10.3969/j.issn.1000-4718.2016.12.027
- VernacularTitle:急性早幼粒细胞患者治疗前后循环内皮细胞的变化及其影响因素的分析
- Author:
Renzhi PEI
;
Jingyi WU
;
Pisheng ZHANG
;
Xuhui LIU
;
Xiaohong DU
;
Dong CHEN
;
Keya SHA
;
Shuangyue LI
;
Junjie CAO
;
Lieguang CHEN
;
Xianxu ZHUANG
;
Peipei YE
;
Zheng FAN
;
Li LIN
;
Shanhao TANG
;
Bibo ZHANG
;
Xiaowei SHI
- Keywords:
Acute promyelocytic leukemia;
Circulating endothelial cells;
Arsenic concentration in urine
- From:
Chinese Journal of Pathophysiology
2016;32(12):2282-2286
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the biological feature of circulating endothelial cells (CECs) in acute promye-locytic leukemia ( APL) patients before and after treatment , and to analyze the relationship between CECs and the clinical characteristics .METHODS: The CECs were sorted from peripheral blood by magnetic-activated cell sorting and then counted by 3-color flow cytometry.The cells were identified by immunofluorescence staining for the expression of CD 146, CD31, CD144, VEGFR-2, CD45 and CD133.The CECs were cultured in vitro, and the tube formation and colony-forming rate were determined .RESULTS:Increased quantity of CECs was observed in CD 34 positive group and group with WBC >10 ×109/L (P<0.05).The quantity of CECs had a significant difference among low risk , medium risk and high risk groups (P<0.05).The positive rate of CD133 and quantity of CECs significantly reduced in 32 APL patients when they gain complete remission after treatment (P<0.05).The amount of tube formation and colony-forming rate were significant-ly reduced after treatment (P<0.05).The ratio of CECs quantity from APL patients after treatment to that before treatment had a negative correlation with arsenic concentration in urine on day 7 during As2O3 treatment (P<0.05).CONCLU-SION:Accurately counting CECs may be helpful for evaluating prognosis and designing treatment strategy .
