Effect of embryonic stem cells-derived neural stem cells on macrophage in vitro

VernacularTitle:胚胎干细胞来源神经干细胞调控巨噬细胞的实验研究
Author: Wen ZHU ; Yaying YUAN ; Fengjun JIAO
Keywords: Neural stem cells; Embryonic stem cells; Macrophage; Phagocytosis; Cytokines
From: Chinese Journal of Immunology 2016;32(12):1741-1744
CountryChina
Language:Chinese
Abstract: Objective:To explore the influence of embryonic stem cells-derived neural stem cells on the proliferation and secretion cytokines of bone marrow-derived macrophages. Methods:Mouse bone marrow derived macrophages were isolated and cultured in L929 medium. After macrophages were treated with NSCs supernatant for 3 days,SRB method was used to detect the proliferation of macrophages. The phagocytosis of macrophages were detected by incubating with RFP-Beads for 1 h. Meanwhile,the expression of TNF-α and IL-1β were detected by ELISA. Results:NSCs were successfully induced from ESC. In control group and NSC group, the proliferation rate of macrophages were 100 % and (126. 29 ± 5. 41)%,the phagocytosis rate were (70. 23 ± 2. 57)% and (90. 32 ± 8. 49)%. Compared to the control group,the levels of IL-6,IL-1βin macrophage treated with NSCs decreased (P<0. 05). Conclusion:ESC-derived NSCs can promote the proliferation and phagocytosis of bone marrow-derived macrophage, and suppress the secretion of pro-inflammatory cytokines.