Comparative studies by different dyeing methods on antiserum preparation against hemorrhagins from Agkistrodon acutus venom
10.3969/j.issn.1000-484X.2016.12.015
- VernacularTitle:在制备尖吻蝮蛇毒出血毒素抗血清中不同凝胶染色方法的比较
- Author:
Chuanbao WEI
;
Jiamin CAO
- Keywords:
Agkistrodon acutus;
Hemorrhagin;
Antiserum preparation;
Dyeing;
Quality test
- From:
Chinese Journal of Immunology
2016;32(12):1793-1796
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to look for a good method for preparation of hemorrhagin antiserum. Methods: Three kinds of hemorrhagins including AaH Ⅰ, AaH Ⅱ, and AaH Ⅳ were purified from Agkistrodon acutus venom according to predecessors's methods and crude AaH Ⅰ, AaH Ⅱ and AaH Ⅳ were obtained. Preparation electrophoresis was used to purify AaH Ⅰ,AaH Ⅱand AaH Ⅳ further. As for an hemorrhagin, six different dyeing methods were used to dye PAGE gel and the gel contained hemorrhagin was obtained respectively. The ground gel contained hemorrhagin was used to immune mice and its antiserum was obtained. Antiserums quality was tested through ELISA test and neutralization of the hemorrhagic activities of corresponding hemorrhagin. Results:Effective IgG concentration in different antiserum was different and effective IgG made through non toxic type protein fast stain reagent kit was higher than others. Conclusion:Non toxic type protein fast stain reagent kit is the best dyeing method among the six dyeing methods.
