Comparison of Effects of Platelet Rich Fibrin and Concentrated Growth Factor on Schwann Cells
10.12007/j.issn.0258-4646.2016.12.008
- VernacularTitle:富血小板纤维蛋白和浓缩生长因子对施万细胞影响的比较
- Author:
Jingyang WU
;
Yanjie BAI
;
Zhiying WANG
- Keywords:
platelet rich fibrin;
concentrated growth factor;
Schwann cells;
nerve growth factor;
cell cycle
- From:
Journal of China Medical University
2016;45(12):1089-1093
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of platelet rich fibrin(PRF)and concentrated growth factors(CGF)using Schwann cells as a pe?ripheral nerve model. Methods A total of 10 healthy volunteers aged 18 to 55 were randomly selected,and 10 mL venous blood was collected un?der aseptic conditions to prepare PRF and CGF. The cells were randomly divided into three groups,control group,PRF group and CGF group. The cell morphology was observed by inverted phase contrast microscope. The cell proliferation was analyzed by MTT assay. The secretion of nerve growth factor in supernatant was detected by enzyme?linked immunosorbent assay. Cell cycle was detected by flow cytometry. Results There was no significant difference of the morphology of cells in each group as observed under inverted phase contrast microscope. MTT results showed that the absorbance values of PRF group and CGF group were significantly higher than those of the control group(P<0.05). The secretion of nerve growth factor in the supernatant were significantly increased(P<0.05). The number of cells in S+G2M phase was significantly increased(P<0.01),but there was no significant difference between PRF group and CGF group. Conclusion Both PRF and CGF can promote proliferation of Schwann cells and increased the amount of nerve growth factor secretion ,but there is no significant difference between PRF and CGF in terms of improving cell proliferation and promoting nerve growth factor secretion.
