PM2.5 Induces Apoptosis in A549 Cells by Stimulating Apoptosis-related Gene Expression
10.12007/j.issn.0258-4646.2016.12.012
- VernacularTitle:PM2.5刺激非小细胞肺癌A549细胞凋亡基因的表达
- Author:
Biao YANG
;
Xinming LI
;
Rong QI
;
Dongmei CHEN
;
Chunling XIAO
- Keywords:
PM2.5;
A549 cell;
cell viability;
apoptosis;
RNA-Seq;
differential genes
- From:
Journal of China Medical University
2016;45(12):1110-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of PM2.5 exposure on A549 cells,and explore the mechanism of toxicity. Method The concentra?tions of PM2.5 exposure were determined utilizing cell viability assay. The morphological characteristics of exposed cell were observed with trans?mission electron microscope. Using big date from RNA?Seq and qRT?PCR,the mechanism was explored. Results The final concentration of PM2.5 exposure was 50μg/cm2;morphological detection showed that chromatin condensation after exposure which was also found on the boundary of nuclear membrane. KEGG pathway analysis and interaction network were finished ,and 8 kinds of apoptosis?related genes were found to be in?volved in the process of damage. Conclusion PM2.5 induces apoptosis in A549 cells by stimulating the changes of apoptosis?related genes ex?pressions.
