Determination of the content and the molecular weight of Mussel Polysaccharide by GPC
10.3969/j.issn.1005-1678.2016.11.003
- VernacularTitle:高效凝胶色谱法测定贻贝多糖含量及分子量
- Author:
Zhongwen HOU
;
Ziang ZHU
;
Jinhua ZHANG
;
Tianjiao ZHANG
;
Fei LIU
;
Peixue LING
- Keywords:
GPC;
Mussel Polysaccharide;
content determination;
molecular weight determination
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(11):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a gel permeation chromatography method for determination of content and molecular weight ( Mw ) of Mussel Polysaccharide.Methods Using GPC method, the sample was separated with TSK-gel GMPWXL(7.8 mm ×300 mm) chromatography column which was set at 35℃.The mobile phase was 0.05 mol/mL NaNO3(including 0.05%Na2N3) and the flow rate was 0.6 mL/min.The detector was RID-20AT. Results The average molecular weight of the polysaccharide of Mytilus coruscus was 1 261 411 and the average content was 88.6%by using of the calibration curves of dextrans.The average molecular weight of the polysaccharide of Mytilus edulis was 1 244 062 and the average content was 87.4%. Conclusion The method established in this paper is simple and rapid, accurate and reproducible, which can be used for the quality control of Mussel Polysaccharide.
