Determination of plasma concentration of mycophenolic acid and mycophenolic acid glucuronide by HPLC
10.3969/j.issn.1005-1678.2016.11.004
- VernacularTitle:高效液相色谱法测定血浆中霉酚酸及其代谢物分化葡萄糖醛麦草酰胺的浓度
- Author:
Chunlan YANG
;
Qin WANG
;
Yong SU
;
Quan XIA
;
Guiyi LIAO
;
Dujuan XU
- Keywords:
mycophelic acid;
mycophenolic acid glucuronide;
renal transplant recipients;
HPLC;
plasma concentration
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(11):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for the determination of mycophenolic acid(MPA), mycophenolic acid glucuronide(MPAG) in plasma.Methods The samples were precipitated with zinc sulphate-methanol solution before injection.Carbamazepine was selected as internal standard,ZORBAX XDB C18 (4.6 mm ×250 mm,5 μm) column was used and the flow rate was 1 mL/min.The mobile phase consisted of methanol-acetonitrile-potassium dihydrogen phosphate buffer solution(gradient elution) .The column temperature was 30℃ and the detective wave length was 254 nm.And then the MPA,MPAG concentration of 32 patients in 7-14 days after renal transplantion were determined.Results The assay was linear within 0.2-50μg/mL for MPA, 2.5-500 μg/mL for MPAG(r>0.999).Absolute recovery rates of MPA,MPAG were more than 80%, the recoveries were between 90%-110%.The intra-day and inter-day RSDs were both lower than 10%.Totally 32 cases of renal transplantion patiens were with mycophenolate mofetil at the dose of 1-1.5 g/d,and MPA in plasma was within the range of 0.32-6.19μg/mL,MPAG in plasma was within the range of 9.52-149.25μg/mL.Conclusion The method is accurate, convenient and rapid, which could be used in the quantitative determination of plasma concentration of MPA,MPAG in renal transplantion patients.
