Effects of miR-30 c on viability and migratory ability of HUVECs by tar-geting PAI-1
10.3969/j.issn.1000-4718.2016.12.012
- VernacularTitle:miR-30c 调控 PAI-1对血管内皮细胞活力和迁移的影响
- Author:
Xiaoyong TAN
;
Mao LUO
;
Peilin LU
;
Jianbo WU
- Keywords:
MicroRNA-30c;
Human umbilical vein endothelial cells;
Plasminogen activator inhibitor-1;
Cell viability;
Cell migration
- From:
Chinese Journal of Pathophysiology
2016;32(12):2199-2204
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of microRNA (miR)-30c on the viability and migratory ability of human umbilical vein endothelial cells (HUVECs) by targeting plasminogen activator inhibitor-1 (PAI-1).METHODS:The HUVECs were transfected with miR-30c mimic and inhibitor or negative control (NC), and then the expression levels of miR-30c, PAI-1 mRNA and protein were detected by RT-qPCR and Western blot.The viability and migratory ability of HUVECs were measured by CCK-8 assay and wound healing test .After bioinformatic analysis, the assessment of miR-30c binding to PAI-1 3’-UTR was carried out using a luciferase reporter gene assay .RESULTS:miR-30c directly down-regu-lated PAI-1 levels by binding to the 3’ UTR seed sequence of PAI-1 mRNA.Furthermore, transfection of a miR-30c mimic down-regulated the expression of PAI-1 at mRNA and protein levels, leading to enhanced migratory ability and viability of the HUVECs.However, transfection of a miR-30c inhibitor up-regulated the expression of PAI-1 at mRNA and protein le-vels, leading to decreased migratory ability and viability .CONCLUSION:Regulation of miR-30c level changes the migra-tory ability and viability of HUVECs by affecting the PAI-1 expression, indicating the involvement of miR-30c in modulating endothelial function .
