The Effect of Hand Twelve Jing-Well Points Bloodletting on Blood-brain Barrier Permeability in PMCAO Rats

VernacularTitle:手十二井穴放血对pMCAO大鼠脑组织血脑屏障通透性的影响
Author: Yucen CHEN ; Zhenguo WANG ; Zelin CHEN
Keywords: Points,Jing-Well; Hand twelve Jing-Well points; Bloodletting therapy; Permanent middle cerebral artery occlusion; Rats; Blood-brain barrier permeability
From: Shanghai Journal of Acupuncture and Moxibustion 2016;35(12):1466-1468
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of hand twelve Jing-Well points bloodletting on blood-brain barrier (BBB) permeability in the early stage of focal cerebral ischemic injury in rats with permanent middle cerebral artery occlusion (pMCAO). Method Male Wister rats were randomized into sham operation, model and bloodletting groups. A model of middle cerebral artery occlusion was made by Longa's method. 2%Evans blue (EB) was injected into the caudal vein at three time points:5, 24 and 72 hrs after model making. EB exudation amount was assessed by measurement of EB absorbance in brain tissue to observe BBB permeability. Result Cerebral EB exudation amount was significantly higher in the model group of rats than in the sham operation group (P<0.01) and significantly lower in the bloodletting group than in the model group (P<0.01). Conclusion Hand twelve Jing-Well points bloodletting can reduce blood-brain barrier permeability in pMCAO rats. Its effect is highly significant at 24 and 72 hrs after ischemia.