Role of TOLL-like receptor 4/MAPKs pathway of renal tissue in diabetic rats by pioglitazone
10.3969/j.issn.1000-484X.2016.12.009
- VernacularTitle:盐酸吡格列酮对糖尿病大鼠肾组织 TOLL受体4/MAPKs信号通路的影响
- Author:
Xin NIE
;
Yiben YU
;
Gebo WEN
- Keywords:
Pioglitazone;
Diabetic nephropathy;
TOLL-like receptor 4/MAPKs signaling pathway
- From:
Chinese Journal of Immunology
2016;32(12):1769-1772
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of TOLL-like receptor4/MAPKs pathway of renal tissue in the diabetic rats treated with piogitazone. Methods:The male Sprague Dawlry rats were randomly selected as the control group (n=8) and experimental group(n=32). The experimental rats were randomly divided into three groups. The expression of ERK1/2,JNK and p38MAPK,and levels of their phosphorylation in kidney were measured by Western blot in control group and experimental group. Results:The expression of TOLL-like receptor 4 in all renal tissue in the diabetic rats were significantlyincreased than those in control group (P<0. 01). Compared with the control group. The activity of p-ERK1/2 and p38MAPK significantly increased in each experimental group (P<0. 05),but the expression level of p-JNK was no statistical significance. TOLL-like receptor 4 could regulatory effect on the phorylation of ERK1/2 and p38MAPK. Compared with the model group,the expression of p-ERK1/2 and p38MAPK significantly decreased(P<0. 05),moreover, these changes were more significant in high-dose treated group ( P<0. 05 ) . Conclusion:Pioglitazone inhibits the expression of TOLL-like receptor 4 of renal tissue in the diabetic rats,and these effects may be related to TOLL-like receptor 4/ERK1/2 and TOLL-like receptor 4/p38MAPK pathways.
