Celastrol increases osteosarcoma cells line HOS lysis by γδ T cells through TRAIL way
10.3969/j.issn.1000-484X.2016.12.011
- VernacularTitle:雷公藤红素经过TRAIL途径增强γδT细胞对骨肉瘤细胞株HOS的杀伤作用
- Author:
Zhaoxu LI
;
Junzhe ZHANG
;
Shengtao WANG
;
Ruiying WANG
;
Jicun TANG
- Keywords:
Osteosarcoma;
γδ T cell;
Immunotherapy;
Celastrol
- From:
Chinese Journal of Immunology
2016;32(12):1777-1780
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether celastrol can increase the cytotoxic activity of γδ T cells against osteosarcoma (OS) cells line HOS through TRAIL way. Methods:The death receptors 4/5 (DR4/5) protein levels in the OS cell lines HOS was in-vestigated by Western blot analysis. Zoledronate ( ZOL) was used to induceγδT cells from PBMCs of healthy volunteers.γδT cell cy-totoxicity against HOS cells was investigated by a standard lactate dehydrogenase release assay ( LDH ) . Results:Celastrol increased DR4/5 protein expression in HOS ( P<0. 05 ) .γδ T cells from PBMCs of healthy volunteers showed cytotoxicity against HOS ( P<0. 05). Following co-culture with HOS pre-treated with celastrol (1 μmol/L) for 24 h,γδ T cells showed significantly higher cytotoxicity against HOS (P<0. 05). The induction of DR4/5 molecules increased lysis of HOS by γδ T cells which was abolished by addition of a blocking TRAIL antibody. Conclusion:Celastrol can enhance γδ T cells'cytotoxic activity against OS cells line HOS through TRAIL way.
