Effect of thalidomide combined with methotrexate on expression of regulatory T cells and IL-6 in rheumatoid arthritis
10.3969/j.issn.1005-1678.2016.11.012
- VernacularTitle:沙利度胺联合甲氨蝶呤对类风湿性关节炎患者外周血调节性T细胞及细胞因子IL－6表达的调节作用
- Author:
Suye WU
;
Ali XUE
;
Pu ZHAO
;
Lingyun LIU
;
Lei JIANG
;
Xiaochun ZHU
- Keywords:
thalidomide;
methotrexate;
rheumatoid arthritis;
regulatory T cells;
IL-6
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(11):47-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of before and after combination therapy of thalidomide and methotrexate (T+M) on regulatory T cells and IL-6 expression profile,and to reveal the mechanism of therapeutic effect of T+M on rheumatoid arthritis.Methods A total of 58 patients with rheumatoid arthritis from August 2014 to August 2016 in the Third Affiliated Hospital of Wenzhou Medical University.The flow cytometry assay and enzyme-linked immunosorbent assay (ELISA) were used to estimate the ratio of peripheral blood regulatory T cells and expression profile of IL-6 in peripheral blood lymphocyte before and after combination therapy of thalidomide and methotrexate .Results After combination therapy of T+M,the ratio of peripheral blood CD25 +Foxp3/CD4 +T cells had a marked increase compared with before combination of therapy of T +M;but the expression of IL-6 had a significant decrease in the group of after combination therapy of T +M compared with before combination therapy.Conclusion The effect of combination therapy of T+M on rheumatoid arthritis is associated with increasing the ratio of regulatory T cells and decreasing the secretion of IL-6 that could regulate T cells to homeostasis.
