Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection.
10.3347/kjp.2016.54.3.375
- Author:
Mu Xin CHEN
1
;
Jia Xu CHEN
;
Shao Hong CHEN
;
Da Na HUANG
;
Lin AI
;
Ren Li ZHANG
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, National Health and Family Planning Commission, Shanghai 200025, PR China. 252120016@qq.com
- Publication Type:Brief Communication
- Keywords:
Angiostrongylus cantonensis;
lateral flow immunoassay;
monoclonal antibody;
antigen detection
- MeSH:
Adult;
Angiostrongylus cantonensis*;
Angiostrongylus*;
Antibodies, Monoclonal;
Diagnosis;
Enzyme-Linked Immunosorbent Assay;
Humans*;
Immunoassay*;
Limit of Detection;
Methods;
Sensitivity and Specificity;
Serologic Tests
- From:The Korean Journal of Parasitology
2016;54(3):375-380
- CountryRepublic of Korea
- Language:English
-
Abstract:
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
