Effects of OSAS model IH on the expressions of YAP and P-YAP in lung cancer cell lines
10.11958/20161061
- VernacularTitle:OSAS模式IH对肺癌细胞系YAP以及P-YAP表达的影响
- Author:
Zhen XUE
;
Lian LI
;
Fangyuan REN
;
Fangxin JIANG
;
Baoyuan CHEN
;
Jie CAO
- Keywords:
sleep apnea,obstructive;
cell hypoxia;
A549 cell;
YAP;
P-YAP;
qRT-PCR;
Western blot
- From:
Tianjin Medical Journal
2016;44(12):1414-1417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of obstructive sleep apnea syndrome (OSAS), OSAS-like intermittent hypoxia (IH)on the expression levels of P-YAP and YAP in A549 lung cancer cell lines. Methods A549 cells were treated with IH exposure ( exposed to 5%O2 for 300 seconds and 21%O2 for 300 seconds) for 1, 3 and 6 h (IH1, IH3, IH6) or normoxia exposure (N group). Quantificational real-time PCR was used to measure the mRNA expression levels of YAP. Western blot assay was used to detect the protein expression levels of YAP and P-YAP. Results The mRNA expression levels of YAP were significantly increased with the increase of IH exposure time points in IH1 (2.50±0.18), IH3 (4.07±0.25) and IH6 (9.18 ± 0.58) groups than those in N group (1.00 ± 0.01) (all P<0.05). The protein expression levels of YAP were significantly increased with the increase of IH exposure time points in IH1, IH3 and IH6 groups than those in N group. The protein expression levels of P-YAP were significantly decreased with the increase of IH exposure time points in IH 1, IH3 and IH6 groups than those in N group. Conclusion YAP cell signaling plays an important role in the process of OSAS-like IH induced tumor development.
