Oxidative Stress Levels in Aqueous Humor from High Myopic Patients.
10.3341/kjo.2016.30.3.172
- Author:
Eun Bi KIM
1
;
Ha Kyoung KIM
;
Joon Young HYON
;
Won Ryang WEE
;
Young Joo SHIN
Author Information
1. Department of Ophthalmology, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. schinn@hanmail.net
- Publication Type:Original Article
- Keywords:
8-Hydroxydeoxyguanosine;
Axial eye length;
Corneal pachymetry;
High myopia;
Malondialdehyde
- MeSH:
Aged;
Aqueous Humor/*metabolism;
Deoxyguanosine/*analogs & derivatives/metabolism;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans;
Male;
Malondialdehyde/*metabolism;
Middle Aged;
Myopia/*metabolism/physiopathology;
*Oxidative Stress;
Refraction, Ocular/*physiology;
Severity of Illness Index
- From:Korean Journal of Ophthalmology
2016;30(3):172-179
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To compare oxidative stress status in the aqueous humor of highly myopic eyes and control eyes. METHODS: Aqueous humor samples were collected from 15 highly myopic eyes (high myopia group) and 23 cataractous eyes (control group) during cataract surgery. Central corneal thickness, corneal endothelial cell density, hexagonality of corneal endothelial cells, and cell area of corneal endothelial cells were measured using specular microscopy. Axial length was measured using ultrasound biometry. 8-Hydroxydeoxyguanosine (8-OHdG) and malondialdehyde levels were measured using enzyme-linked immunosorbent assay. RESULTS: 8-OHdG level was lower in the aqueous humor of myopic patients than in that of control group (p = 0.014) and was positively correlated with central corneal thickness and negatively correlated with axial length (r = 0.511, p = 0.02; r = -0.382, p < 0.001). There was no correlation between 8-OHdG level and corneal endothelial cell density, hexagonality, or cell area. Malondialdehyde level did not show any correlation with any parameters evaluated. CONCLUSIONS: 8-OHdG might be a sensitive biomarker for evaluating oxidative stress status in the eye. Oxidative stress level was lower in the aqueous humor of highly myopic eyes compared to that in control eyes, which indicates lower metabolic activity in these eyes.
