Study on Anti-influenza Virus H1N1 Activity of Main Ingredients from Microemulsion Extract of Compound Longqincaoin Vitro
10.3969/j.issn.1005-5304.2017.01.013
- VernacularTitle:复方龙芩草微乳提取液有效成分体外抗H1N1流感病毒的药效研究
- Author:
Xiaoyan LIU
;
Yan LI
;
Shuai LIU
;
Weifei LI
;
Ligang GU
;
Xiaoting LIU
;
Hong DU
- Keywords:
CompoundLongqincao;
microemulsion extract;
uniform design;
H1N1 influenza virus;
A549 cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(1):49-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of main ingredients from microemulsion extract of Compound Longqincao in vitro on anti-influenza virus H1N1 activity; To analyze effects of main ingredients from microemulsion extract on influenza virus inhibition rate.Methods Uniform design was used to conduct the experiment. MTT method was used to detect the effect rate (ER) of anti-influenza virus H1N1 on A549 cells. Setting ER as the index, Minitab17 software was used to establish mathematical model to come up with regression equations of all factors. The effects of ingredients on ER were analyzed and the efficient composition ratio of the optimum anti-influenza virus H1N1 was chosen.ResultsIn the compound compatibility, baicalin showed the most obvious antivirus activity, and licorice glycosides had certain inhibition effects on pathological changes of cells. Five ingredients had coordinative or controlled relation with ER. When per milliliter liquids containing licorice glycosides, baicalin, leucine, aspartic acid, glutamic acid was 13.94μg, 49.44μg, 0.23 mg, 1.25 mg, and 2.50 mg, ER was the best. ER was 85.34%±4.72% after verification.ConclusionThe optimized combination of main ingredients from microemulsion extract of Compound Longqincaocan better play a role in anti-influenza virus H1N1.
