Dynamic Expression of IL-1β, IL-4, IFN-γ and TGFβ1 in Serum and Colon Tissue in Rats with Ulcerative Colitis of Spleen-Kidney Yang Deficiency Type
10.3969/j.issn.1005-5304.2017.01.015
- VernacularTitle:相关炎性因子在脾肾阳虚型溃疡性结肠炎模型大鼠血清和结肠组织的动态表达
- Author:
Lanjuan HE
;
Xiangdong ZHU
;
Yan WANG
;
Tingting GUO
;
Di WANG
- Keywords:
ulcerative colitis;
spleen-kidney yang deficiency;
IL-1β;
IL-4;
IFN-γ;
TGF-β1;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change rules of expressions of IL-1β, IL-4, IFN-γ and TGF-β1 in serum and colon tissue in rats with ulcerative colitis of spleen-kidney yang deficiency type; To explore the action in the progress of ulcerative colitis of spleen-kidney yang deficiency type.MethodsComposite method was used to establish rat models with ulcerative colitis of spleen-kidney yang deficiency type. 75 Wistar rats were randomly divided into blank group, model 3 d group, model 7 d group and model 21 d group. ELISA was used to test the levels of IL-1β, IL-4, IFN-γ and TGF-β1 in serum and colon tissue.Results Compared with the blank group, contents of IL-1β and IFN-γ in model group increased significantly (P<0.05,P<0.01), and the contents of IL-4 and TGF-β1 content decreased (P<0.05,P<0.01),especially in the model 7 d group (P<0.01).ConclusionThe pro-inflammatory factor IL-1β, IFN-γ and anti-inflammatory factor IL-4 and TGF-β1 play an important role in rats with ulcerative colitis of spleen-kidney yang deficiency type.
