Regulatory mechanisms of K-ras to girdin in COS7 cells and expression of K-ras and girdin in colorectal carcinoma
10.3969/j.issn.1000-4718.2017.02.017
- VernacularTitle:K-ras 对 girdin 蛋白表达的调控机制及二者在结直肠癌中的表达
- Author:
Zhihao WU
;
Haibin LIU
;
Min ZHENG
- Keywords:
K-ras;
Girdin;
Colorectal cancer
- From:
Chinese Journal of Pathophysiology
2017;33(2):297-301
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate molecular regulatory mechanism of K-ras to girdin protein in COS7 cells and expression of K-ras and girdin in colorectal carcinoma tissues .METHODS:The lentiviral vector carrying K-ras gene was constructed and transfected in the COS 7 cells.The expression of K-ras, girdin proteins and other related proteins in COS 7 cells and colorectal carcinoma tissues was observed by Western blot .RESULTS:The COS7 cells with K-ras over-expres-sion showed an irregular cell morphology .The results of Western blot indicated that the downstream signal protein levels of p-ERK1/2, p-Stat3 and girdin were significantly increased in the COS 7 cells with K-ras over-expression.Transfection with the K-ras siRNA into the COS7 cells significantly reduced the protein levels of p-ERK1/2, p-Stat3 and girdin.In the color-ectal carcinoma tissues (7 cases), 5 cases had higher expression of K-ras and girdin compared with pericarcinous tissues . CONCLUSION:K-ras regulates girdin expression through the signal pathway of K-ras-ERK1/2-Stat3-girdin.
