Effect of miRNA-21 on protection of PC12 cells from oxygen-glucose dep-rivation damage
10.3969/j.issn.1000-4718.2017.02.028
- VernacularTitle:miRNA-21减轻氧糖剥夺对 PC12细胞的损伤
- Author:
Jian YU
;
Yang ZHANG
;
Yingjiu CHAO
;
Ge GAO
;
Yu CHEN
;
Daqun GU
- Keywords:
MicroRNA-21;
PI3K/AKT signaling pathway;
Oxygen-glucose deprivation;
PC12 cells
- From:
Chinese Journal of Pathophysiology
2017;33(2):361-364
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of microRNA ( miRNA)-21 on the PC12 cells with hypoxic-ischemic damage.METHODS:The PC12 cells were cultured in vitro, and the cell model of oxygen-glucose deprivation (OGD) was established .In accordance with the following requirements , the cells were randomly divided into control group , OGD group, negative control sequence +OGD group, miRNA-21 inhibitor +OGD group and miRNA-21 mimic+OGD group. The effects and mechanism of miRNA-21 on the protection of PC12 cells from OGD damage were determined by CCK-8 as-say, real-time PCR and Western blot .RESULTS:Decrease in the expression of miRNA-21 by transfection with miRNA-21 inhibitor inhibited the viavility of the PC 12 cells subjected to OGD damage .Increase in the expression of miRNA-21 by transfection with miRNA-21 mimic promoted the viability of the PC 12 cells subjected to OGD damage .It was further con-firmed that miRNA-21 promoted the AKT phosphorylation in OGD-damaged PC12 cells.CONCLUSION: miRNA-21 sig-nificantly increases the viability of PC 12 cells subjected to OGD damage , which may be related to the activation of PI 3K/AKT signaling pathway .
