Evaluation of immune effects of recombinant norovirus GⅠ. 1 and GⅡ. 4 virus-like particles
10.3760/cma.j.issn.0254-5101.2016.12.009
- VernacularTitle:重组诺如病毒GⅠ.1和GⅡ.4型类病毒颗粒免疫效果初步评价
- Author:
Zhijing MA
;
Fang TANG
;
Xuefeng ZHANG
;
Jing ZHANG
;
Junwei HOU
;
Shi CHEN
;
Qiming LI
- Keywords:
Norovirus (NoV);
Virus-like particles (VLPs);
Histo-blood group antigen (HBGA)
- From:
Chinese Journal of Microbiology and Immunology
2016;36(12):930-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immune effects of virus-like particles ( VLPs) of VP1 pro-teins derived from norovirus GⅠ. 1 and GⅡ. 4 genotypes expressed in Hansenula polymorpha expression sys-tem. Methods SDS-PAGE and Western blot assay were performed to detect the purity of GⅠ. 1 and GⅡ. 4 VP1 proteins after purification. Morphologies of the recombinant VLPs were observed under transmission electron microscopy ( TEM) . Sizes and distributions of the VLPs were analyzed by dynamic light scattering analyzer. BT50(50% of blocking titer) was detected by HBGA (histo-blood group antigen) blocking assay in BALB/c mice immunized with different regimens. Results SDS-PAGE analysis of the purified recombinant GⅠ. 1 and GⅡ. 4 VP1 proteins showed that their purity were greater than 90%. Western blot assay con-firmed the specific bands of VLPs. TEM images showed that the sizes of purified GⅠ. 1 and GⅡ. 4 VP1 VLPs were at a mean diameter of 30-50 nm with clear border and high homogeneity, which was similar to that of wild virus. BT50 significantly increased in the groups, in which Al( OH) 3 was used as adjuvant. Con-clusion Animal studies have shown that administration of GⅠ. 1 and GⅡ. 4 VP1 VLPs in the presence of Al( OH) 3 induces detectable HBGA-blocking antibody, indicating that GⅠ. 1 and GⅡ. 4 VP1 VLPs are promising candidates for norovirus vaccine.
