The expression of Bcl-2 and Bax proteins and their significance with the atrophy of parotid gland cells in rats
10.3969/j.issn.1001-3733.2016.06.003
- VernacularTitle:Bcl-2及Bax在大鼠腺体萎缩过程中的表达及临床意义
- Author:
Hong GAO
;
Jinhua ZUO
;
Lifang WANG
;
Yuhong ZHU
;
Yong YANG
;
Fang WANG
;
Bing SONG
;
Yulong MAO
- Keywords:
Parotid gland;
Apoptosis;
Bcl-2;
Bax
- From:
Journal of Practical Stomatology
2016;32(6):758-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and correlation of Bcl-2 and Bax in the parotid gland after leading duct ligation in rats. Methods:Atrophy of the right parotid was induced by ligating the right main duct of 72 rats. Immunohistochemical labelling was performed to study the expression of Bcl-2 and Bax 1, 3, 5, 7, 14, 21, 30, 60, 90, 150 and 180 days after duct ligation. Results:7 d after duct ligation most acinar cells disappeared. The distribution of Bcl-2 and Bax protein in normal parotid was in cytoplasm with unifo-maity. Bcl-2 and bax higher expression was identified in the gland at all time points. The expression of Bcl-2 protein was significantly in-creased and reached the peak at 21 d after duct ligation. More Bax-positive acinar cells on day 3 were observed, then the expression of Bcl-2 and Bax protein was descended. Higher Bcl-2/Bax ratio was identified at 1-21 d, then descended. Conclusion:The expression of Bcl-2 and Bax is associated with the atophy of the parotid glad after rat parotid duct ligation.
