Heavy ion irradiation inhibits the expression of invasion and metastasis associated gene RhoC in tongue squamous cell carcinoma Tca8113 cells

VernacularTitle:重离子辐照对舌癌Tca8113细胞中转移相关基因RhoC的作用
Author: Chunqing LI ; Zhenghu FENG ; Xin LI ; Xuan ZHANG ; Zhijie LI
Keywords: Heavy ion beam; Tongue squamous cell carcinoma; RhoC
From: Journal of Practical Stomatology 2016;32(6):787-790
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of heavy ion beam on the invasion and metastasis associated gene RhoC expression in tongue squamous cell carcinoma Tca8113 cells. Methods:Real-time PCR and Western-blot were used to detect the expression of RhoC mRNA and RhoC protein respectively after Tca8113 cells were irradiated by heavy ion beams of 12 C6+. Results:The expression levels of RhoC mRNA in the experimental group were lower than that in the control group(P<0. 05) except for the levels at 12 h after 2 Gy and 4 Gy 12 C6+ irradiation. In addition, the expression levels of RhoC protein increased in a dose-dependent manner at 12 h af-ter irradiation, reaching a peak at 4 Gy, and subsequently decreased with the increase of irradiation dose. The expression level of RhoC protein was consistent with that of RhoC mRNA. Conclusion:Heavy ion beam of 12 C6+ may inhibit RhoC gene expression in Tca8113 cells.