Effects ofWenyujin Essential Oil on tau Protein Phosphorylation in Mice with Aβ-induced Alzheimer Disease through PI3k/Akt Pathway
10.3969/j.issn.1005-5304.2017.01.012
- VernacularTitle:温郁金挥发油通过PI3K/Akt信号途径对阿尔茨海默模型小鼠tau蛋白磷酸化表达的影响
- Author:
Yue QI
;
Wenyan QIN
;
Kai KANG
;
Hong JIANG
;
Zhao LI
;
Yabin WANG
;
Dong JIA
- Keywords:
Wenyujin essential oil;
Alzheimer disease;
tau protein;
PI3K/Akt;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects ofWenyujinessential oil on tau protein phosphorylation in mice with Alzheimer Disease (AD); To discuss the relevant mechanism of action.MethodsSixty Kunming mice were divided into six groups randomly: sham-operation group, model group,Wenyujin essential oil low- and high-dose group, donepezil group andShenzhiling group.β-Amyloid protein (Aβ25-35) was infused into the hippocampal of mice. Mice in each group were given relevant medicine for gavage for 15 d. After the last administration, immunohistochemistry was used to detect the expressions of tau protein phosphorylation points (Ser 404 and Thr 231). Western blot was used to detect tau protein phosphorylation points and the expression of PI3K/Akt protein. Results Compared with sham-operation group, tau protein phosphorylation points in the model group increased (P<0.05), but the phosphorylation levels of PI3K and Akt decreased significantly (P<0.05). Compared with the model group, tau protein phosphorylation (Thr231 and Ser404) inWenyujin essential oil high-dose group decreased significantly (P<0.05), and the phosphorylation levels of PI3K and Akt increased(P<0.01). ConclusionWenyujinessential oil can inhibit tau protein phosphorylation in mice. The possible mechanism may be related to the PI3K/Akt signal pathway.
