Acetyltransferase MORF regulates osteogenic differentiation potential of periodontal ligament stem cells
10.3969/j.issn.1001-3733.2016.06.007
- VernacularTitle:牙周膜干细胞中乙酰基转移酶MORF调控成骨的研究
- Author:
Lin YUAN
;
Jin SUN
;
Feng CHENG
;
Zhengyi YANG
;
Yina CAO
;
Guangsi PAN
;
Jun QIAN
;
Enliang HE
;
Han WANG
- Keywords:
Periodontal ligament stem cells( PDLSCs);
Periodontitis;
Acetyltransferase;
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2016;32(6):778-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare acetyltransferase MORF level in periodontal ligament stem cells( PDLSCs) derived from healthy individuals ( H-PDLSCs) with those derived from the individuals with periodontitis ( P-PDLSCs ) . And to determine the effect of MORF on the osteogenic differentiation potential of PDLSCs. Methods: Human H-PDLSCs and P-PDLSCs were cultured and cloned with limited dilution method. H-PDLSCs were stimulated by LPS, TNF-α, IL-β and the mix of the 3 inflammatory factors to imitate inflammatory environment ( IP-PDLSCs ) . Quantitative RT-PCR and Western Blot were applied to examine different expression of MORF in H-PDLSCs and P-PDLSCs. Western Blot was applied to detect expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red staining were applied to determine osteogenic differentiation potential of H-PDLSCs with MORF knock-down. Results:Quantitative RT-PCR and Western Blot showed lower expression of MORF in P-PDLSCs compared with H-PDLSCs( P<0. 05). Western Blot revealed lower expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red stai-ning indicated osteogenic differentiation potential was inhibited in H-PDLSCs with MORF knockdown(P<0. 05). Conclusion: Peri-odontitis can suppress the expression of MORF in PDLSCs and inhibite the osteogenic differentiation potential of PDLSCs.
