Influences of protein kinase Cβ inhibitor LY333531 on oxidative injury and apoptosis of SH-SY5Y cells induced by fluorosis
10.3760/cma.j.issn.2095-4255.2017.02.004
- VernacularTitle:蛋白激酶Cβ抑制剂LY333531对氟诱导人神经母瘤细胞SH-SY5Y氧化损伤及凋亡的影响
- Author:
Chengmin DENG
;
Liang ZHAO
;
Longchun TAN
;
Zhizhong GUAN
- Keywords:
Fluoride;
Reactive oxygen species;
Apoptosis
- From:
Chinese Journal of Endemiology
2017;36(2):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influences of protein kinase Cβ (PKC3) inhibitor LY333531 on oxidative injury and apoptosis of SH-SY5Y cells induced by fluorosis.Methods The SH-SY5Y cell model of fluorosis was established,and the experiment was divided into three groups:control group [0.0 mmol/L sodium fluoride (NaF) and 0.0 μmol/L LY333531],the fluoride group (0.5 mmol/L NaF and 0.0 μmol/L LY333531),and the PKCβ inhibitor group (0.5 mmol/L NaF and 0.2 μmol/L LY333531),n =3.Flow cytometry was used to detect the changes of reactive oxygen species (ROS) and apoptosis rate,fluorescent probe technique was used to detect mitochondrial membrane potential after each group for 48 h.Results Compared with the control group [(3.32 ± 0.29) × 103,0.60 ± 0.09,(7.58 ± 1.20)％],the level of ROS [(5.99 ± 0.32) × 103] was increased,mitochondrial membrane potential (0.28 ± 0.06) was decreased,and the apoptosis rate [(18.00 ± 2.32)％] was increased in the fluoride group (all P ＜ 0.05);compared with the fluoride group,the level of ROS [(5.12 ± 0.25) × 103] was decreased,mitochondrial membrane potential (0.42 ± 0.03) was increased,and the apoptosis rate [(11.79 ± 0.70)％] was decreased in the PKCβ inhibitor group (all P ＜ 0.05).Conclusions Excess fluoride could cause oxidative damage and apoptosis in cells.PKC3 inhibitor LY333531 has a protective effect in oxidative damage and apoptosis by fluorosis.
