Effect of apatinib on cell proliferation, migration and apoptosis in pancreatic cancer cell line AsPC-1
10.3760/cma.j.issn.1674-1935.2017.01.004
- VernacularTitle:阿帕替尼对胰腺癌细胞株AsPC-1增殖、迁移和凋亡的影响
- Author:
Xiaoxia GU
;
Jie LI
;
Meihong WU
;
Xiaobo PENG
;
Xianbao ZHAN
- Keywords:
Apatinib;
Pancreatic neoplasms;
Cell proliferation;
Cell movement;
Apoptosis
- From:
Chinese Journal of Pancreatology
2017;17(1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of apatinib on the proliferation,apoptosis and migration of pancreatic cancer cell line AsPC-1 in vitro.Methods Pancreatic cancer AsPC-1 cells were treated by apatinib in different concentrations.Cell proliferation and apoptosis were measured by CCK-8 and flow cytometry,and the effect of apatinib on cell migration ability was observed by wound healing assay.Results In control and 10,20,30,40 and 50umol/L apatinib treatment group,the inhibitory rates of AsPC-1 cells were 0,(1.45 ±0.68)％,(16.92±0.70)％,(23.84±0.84)％,(34.35±1.55)％ and (37.33± 0.81) ％,respectively.Cell proliferation was obviously inhibited by apatinib as the concentration increased,and the differences were statistically significant (P ＜ 0.05).In control and 20,40 umol/L apatinib treatment group,the apoptotic rates were (9.44 ± 0.18) ％,(16.62 ± 0.19) ％ and (25.42 ± 0.41) ％,respectively.Number of apoptotic cells was obviously increased by apatinib as the concentration increased,and the differences were statistically significant (P ＜ 0.05).In control and 20,40 umol/L apatinib treatment group,the migration ability was (29.5 ± 0.7) ％,(17.4 ± 0.9) ％ and (6.6 ± 0.5) ％,which was greatly decreased as the concentration increased,and the differences were statistically significant (P ＜ 0.05).Conclusions Apatinib can effectively inhibit the proliferation and migration of pancreatic cancer AsPC-1 cells and induce apoptosis.
