Screening of Alzheimer's disease-associated gene expression changes through peripheral blood monocytes extracted gene microarray analysis in patients with Alzheimer's disease

VernacularTitle:阿尔茨海默病患者外周血单核细胞的疾病相关基因筛查
Author: Li TIAN ; Juan FENG ; Quan SHI ; Weishuang XUE ; Weiyu TENG
Keywords: Alzheimer disease; Monocytes; Amyloid beta-protein; Oligonucleotide array sequence analysis
From: Chinese Journal of Geriatrics 2016;35(12):1267-1270
CountryChina
Language:Chinese
Abstract: Objective To screen the amyloid protein-β(Aβ)degradation-associated genes through peripheral blood monocytes(PBMC)-extracted gene microarray analysis in patients with Alzheimer's disease(AD).Methods PBMC were isolated from blood of elderly AD patients versus age-matched healthy individuals(control).The cDNA(mRNAs)were analyzed using gene microarray.And real-time quantitative polymerase chain reaction detection and enzyme activity analysis were used to verify the primary outcome of gene chip.Results The expression of cathepsin D mRNA in peripheral blood monocytes was 104.70±15.96 in AD patients as compared with the control group 49.86±5.19,and the activity of cathepsin D was (22 620 ± 1 389) RFU in AD versus (32 210 ± 2 284) RFU in control (both P＜0.05).Conclusions The results suggest that the decreased levels of cathepsin D could be the stage markers related to the pathophysiology of AD process.Based on the microarray data,we select cathepsin D genes for further study.