IAP-1 gene silencing enhanced the radiosensitivity of liver cancer SMMC-7721 cells
10.3760/cma.j.issn.1007-8118.2017.02.015
- VernacularTitle:沉默cIAP-1基因对肝癌SMMC-7721细胞放疗敏感性的影响
- Author:
An SHEN
;
Xuelian WANG
;
Houfa NING
;
Bingqi MA
;
Shiping LI
- Keywords:
Liver cancer;
Cellular inhibitor of apoptosis protein 1 (cIAP-1);
Radiosensitivity;
Apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cellular inhibitor of apoptosis protein1 (cIAP-1) gene on the radiosensitivity of SMMC-7721 cells.Methods We silenced cIAP-1 expressions by the shRNA technology,and then we detected the changes of cell proliferation,cell cycle and cell apoptosis by CCK8 as-say,Western blot,qRT-PCR and flow cytometry after the radiotherapy.Results The cell proliferation rate of liver cancer SMMC-7721 cells at different radiation doses of 1 Gy,4 Gy,7 Gy and 10 Gy was detected.Comparing with control group (pGCsi-H1-control),the cell proliferation in cIAP-1 silencing group (pGCsiH1-shRNA) was significantly reduced at various radiation doses,and the effect was dose-dependent (P ＜ 0.05).G1/G0 phase arrest was observed after radiation (P ＜0.01),and the proportion of cells in S phase was significantly reduced compared with control (P ＜ 0.01).Compared with control group,G1/G0 phase arrest was detected (P ＜ 0.05),and the percentage of cell apoptosis was increased significantly in cIAP-1 silencing group (P ＜ 0.05).Conclusion cIAP-1 silencing can enhance the radiosensitivity of liver cancer cells,and inhibit cell proliferation by promoting the anti-tumor effect of radiation.
