Effects of interleukin-35 on proliferation and migration of HepG2 cell line
10.3760/cma.j.issn.1007-8118.2017.01.005
- VernacularTitle:白细胞介素-35对肝癌细胞株HepG2增殖和迁移能力的影响
- Author:
Haiming WANG
;
Haorun LIU
;
Li XIAO
;
Hongqiang ZHAO
;
Yu GAO
;
Hongtao CUI
;
Weimin LI
- Keywords:
Interleukin-35;
Hepatocellular carcinoma;
Proliferation activity;
Invasion;
Migration
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of interleukin-35 (IL-35) on proliferation,invasion and migration of a HepG2 cell line in vitro.Methods Enzyme-linked immunosorbent assay (ELISA) was used to determine peripheral blood expression levels of IL-35 in hepatocellular carcinoma patients and in normal healthy subjects.The effects of exogenous IL-35 on cell proliferation activity of HepG2 in vitro were measured using CCK-8 assay.The transwell invasion and scratch test were used to study the invasive and migration abilities.Results The content of IL-35 protein in hepatocellular carcinoma patients (HCC) was significantly higher than in healthy individuals [(118.45 ±28.66) ng/L vs (39.56 ± 11.15) ng/L,P ＜0.05].Exogenous IL-35 significantly promoted proliferation,invasion and migration abilities of HepG2 cells in vitro (P ＜ 0.05).Conclusions The level of IL-35 was elevated significantly in hepatocellular carcinoma patients.IL-35 had biological characteristics of promoting HCC cell proliferation,invasion and migration.
