Role of Akt and SRC Pathways in Exosome-mediated Migration of Homologous Lung Adenocarninoma Cells

VernacularTitle:Akt和SRC在Exosomes促进同源肺癌细胞迁移中的作用
Author: Shilin XIE ; Jinglei QU ; Yibo FAN ; Xiaofang CHE ; Kezuo HOU ; Xiujuan QU ; Yunpeng LIU ; Xiaonan WANG ; Jian KANG ; Xuejun HU
Keywords: Exosomes; migration; lung cancer; Akt; SRC
From: Journal of China Medical University 2017;46(4):294-297
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of Exosomes derived from lung cancer cells on the migration of secretory cells and homologous tumor cells and to explore the role of PI3K/Akt and SRC signaling pathways in this process.Methods Exosomes were isolated from the supematant post density gradient centrifugation of A549,lung cancer cells.Morphology of the Exosomes was studied using transmission electron microscopy.Protein expression was analyzed using Western blotting.Cell migration was analyzed by a transwell assay.Results The double-membrane-bound Exosomes appeared as discal-shaped structures,30-100 nm in diameter.Western blotting showed that CD9 was abundant in the Exosomes.The Exosomes promoted the migration of A549 cells and their homologous tumor cells,HCC827 in a dose-dependent manner,accompanied by the activation of Akt and SRC.Conclusion The Exosomes derived from A549 (lung cancer) cells promote the migration of the secreting cells and the homologous tumor cells.The mechanism may be correlated with the activation of Akt and SRC.