The Mechanism of Neuroprotective Effects of Puerarin for the Treatment of Acute Spinal Ischemia-reperfusion Injury in Rats
10.12007/j.issn.0258-4646.2017.04.007
- VernacularTitle:葛根素治疗急性脊髓缺血再灌注损伤大鼠的神经保护机制
- Author:
Xianglu JI
;
Feng TIAN
;
Bin WANG
;
Wanan XIAO
- Keywords:
puerarin;
spinal ischemia-reperfusion;
cyclin-dependent kinase 5;
cyclin B kinase inhibitor;
rat
- From:
Journal of China Medical University
2017;46(4):313-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of neuropmtective effects of puerarin for the treatment of acute spinal ischenia-reperfusion injury in a rat model.Methods Acute spinal ischemia-reperfusion injury was induced via aortic occlusion in 28 male Sprague-Dawley rats.The animals were randomly divided into four groups,as follows:group negative contrast (NC sham operation),group positive control group (IR+ S ischemia/reperfusion + saline),group puerarin (IR+P ischemia/reperfusion + puerarin),group mscovitine (IR+R ischemia/reperfusion + roscovitine).The motor function,spinal infarction volume,apoptosis indices,and CDK5 and P25 activities were examined.Results Spinal ischemia-reperfusion caused the injury of the spines and was associated with motor deficit,elevation of CDK5 and P25 activities,and increase in the spinal apoptosis and spinal infarction volume.Puerarin improved motor function and decreased apoptosis,spinal infarction volume,and CDK5 and P25 activities.Conclusion The findings of the present study indicated that puerarin treatment-mediated reduction of spinal injury was associated with the inhibition of CDK5 and P25,and that the inhibition was one among the neuroprotective mechanisms of puerarin against acute ischemia/reperfusioninduced spinal injury in rats.
