MiR-31 inhibit the invasion of breast cancer by Dock180
10.13315/j.cnki.cjcep.2017.03.002
- VernacularTitle:miR-31通过结合Dock180抑制乳腺癌细胞侵袭
- Author:
Shuxian ZHENG
;
Xuemei SUN
;
Ruige WANG
;
Lihong SHI
;
Baogang ZHANG
- Keywords:
breast neoplasm;
miR-31;
Dock180;
invasion
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the correlation of Dock180 and miR-31 expression in breast cancer cells,and to observe the effect of miR-31 on the invasion of breast cancer cells by Dock180.Methods MiR-31 was transfected into breast cancer cells by liposome transfection technique.The actual binding site of miR-31 to the 3'-untranslated region of Dock180 was confirmed through luciferase assay.Western blot was performed to detect the expression of Dockl80 and other related proteins.Real-time PCR was used to measure the expression of Dock180.Matrigel invasion were performed to detect the invasion of breast cancer cell lines with miR-31 increased.Resuits The protein levels of Dock180 in breast cancer cell lines negatively correlated with miR-31 expression,and Dock180 was directly targeted by miR-31.Dock180 downregulation and miR-31 overexpression reduced breast cancer cells invasion.Conclusion Dock180 modulated by miR-31 plays an important function in breast cancer cell lines invasion.
