Study of predictive value for detection of high-grade cervical intraepithelial neoplasia by p16/Ki-67 dual-stained liquid-based cytology
10.13315/j.cnki.cjcep.2017.03.011
- VernacularTitle:细胞免疫化学p16/Ki-67双染预测子宫颈上皮内高级别瘤变
- Author:
Yongxiang YIN
;
Hua ZHAO
;
Jie LIANG
;
Jinqiu ZHANG
;
Min ZHAO
;
Jiajun WANG
- Keywords:
cervical intraepithelial neoplasia;
p16/Ki-67 dual staining;
immunocytochemistry;
HR-HPV
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(3):282-286
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study of predictive value for detection of high-grade cervical intraepithelial neoplasia (CIN2 +) by p16/Ki-67 dual-stained liquid-based cytology.Methods Random collection of 123 women including 103 samples of atypical squamous cell of undetermined significance (ASC-US) and above with results of high-risk human papillomavirus (HR-HPV)testing and cervical biopsy,20 samples of negative for intraepithelial lesion or malignancy (NILM) by using immunocytochemical p16/Ki-67 dual-stained and the morphology assessment.Results In normal control group,the expression of p16/Ki-67 dual-stained in squamous epithelial cells were negative.Sensitivity of p16/Ki-67 dual-staind cytology for biopsy-confirmed CIN2 + was 66.67％ (ASC-US),91.67％ (LSIL) and 92.86％ (HSIL),specificity rates were 95.92％ (ASC-US),95.00％ (LSIL) and 0 (HSIL),positive predictive value were 50.00％ (ASC-US),91.67％ (LSIL) and 92.86％ (HSIL),negative predictive value were 97.92％ (ASC-US),95.00％(LSIL) and 0 (HSIL),respectively.Condusion p16/Ki-67 dual-stained cytology are improved obviously the predictive value for detection of CIN2 +,p16/Ki-67 dual-stained cytology may efficiently complement HPV-based screening programs to prevent cervical cancer.
