NET-1 promote the angiogenesis of cervical squamous cell carcinoma by activating NF-kappa B signaling pathway
10.13315/j.cnki.cjcep.2017.03.008
- VernacularTitle:NET-1活化NF-κB信号途径促进子宫颈鳞癌微血管生成
- Author:
Yuting ZHANG
;
Wenhui ZHANG
;
Xiujuan XIONG
;
Xiaodong KUANG
;
Enlin SONG
- Keywords:
cervical neoplasm;
squamous carcinoma;
NET-1;
NF-κB;
VEGF;
angiogenesis
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the association of NET-1 expression with the angiogenesis of cervical squamous cell carcinoma and the related molecular mechanism.Methods Immunohistochemistry MaxVision was used to detect the expression of NET-1,NF-κB p65 and VEGF in 80 cases of cervical squamous cancer tissue and 10 cases of chronic cervicitis tissue.The microvascular density (MVD) was assessed by labeling endothelia with CD34-antibody and counted according to Weinner's standard.The eukaryotic expression plasmid pCMV6-NET-1 was transfected into cervical squamous cancer cells by cationic liposome in order to obtain over expression of NET-1 gene.In order to inhibit the activation of NF-kappa B,cervical squamous carcinoma cells were treated with NF-kappa B specific inhibitor PDTC.The protein expression in SiHa cells was assessed by Western blotting.Results The expression level of NET-1 in the cervical squamous carcinoma tissue was significantly higher than that in the tissue of control group.NET-1 expression was positively correlated to the MVD,NF-κB p65 and VEGF expression,respectively.Transient transfection of NET-1 gene significantly increased the nuclear expression of NF-κB p65 and cytoplasm expression of VEGF in the SiHa cells.However,pretreatment of SiHa cells with NF-inhibitor PDTC significantly inhibited NET-1 induced upregulation of VEGF expression.Conclusion NET-1 promotes the angiogenesis of cervical squamous carcinoma through the activation of the NF-κB signaling which upregulate the expression of VEGF in the cancer cells.
