The hydride generation atomic fluorescence spectrophotometry method for determination of urinary arsenic
10.3760/cma.j.issn.2095-4255.2017.04.014
- VernacularTitle:氢化物发生原子荧光分光光度法检测尿中砷的方法学探讨
- Author:
Yuehua PAN
;
Yiyanwen HUANG
;
Dancheng YAO
;
Nianheng ZHANG
;
Jing GAO
;
Yu YANG
;
Danya SHE
;
Honglong WU
- Keywords:
Hydride generation atomic fluorescence spectrometry;
Urine;
Arsenic
- From:
Chinese Journal of Endemiology
2017;36(4):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply hydride generation atomic fluorescence spectrophotometry (HG-AFS method) in urinary arsenic detection,and to provide a better,newer and more convenient detection method for quantitative analysis of urinary arsenic.Methods According to the Guide to Develop Biological Sample Inspection Method(WS/T 68-1996) and Guide for Establishing Occupational Health Standards-part 5:Determination Methods in Biological Materials (GB/T 210.5-2008),HG-AFS method was established to detect arsenic content in urine after modification of the method for sample pretreatment,and to verify the linear range of standard curve and linearity,detection limit,precision,accuracy,stability of the sample,and to compare the experimental results of HG-AFS method with those of standard methods of WS/T 28-1996 and Determination of Arsenic in Urine by Cyanide Generation Atomic Fluorescence Method (WS/T 474-2015).Results The HG-AFS method linear range was from 0-100 μg/L,the correlation coefficient r =0.999 9,the detection limit was 0.07 μg/L,the precision was 1.96％-3.97％,and the recovery rate was 95.1％-105.0％.There was no statistical significance between HG-AFS method,the standard of WS/T 28-1996 or WS/T 474-2015 methods (t =1.539,0.353,all P ＞ 0.05).Conclusion The new method is superior to the current detection method owing to its low detection limit,high precision,good accuracy,and wide linear range.
