Methods of blood glucose measurement for early-stage severely burned rats
10.3760/cma.j.issn.1674-6090.2016.06.010
- VernacularTitle:严重烧伤后早期大鼠血糖测量方法探讨
- Author:
Lu ZUO
;
Weiren LI
;
Jiaxiang LU
;
Xiang YU
- Keywords:
Burn;
Rat;
Blood glucose
- From:
Chinese Journal of Endocrine Surgery
2016;10(6):483-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differences of blood glucose detected by four methods with different instruments and specimen types at early stage in severely burned rats.Methods 24 Sprague-Dawley (SD) rats were randomly divided into two groups:control group 1 (Sham scald group,n=8) and scald injury group 1 (n=16).Blood samples of scald injury group 1 were collected at 12,and 24 hours after scald (n=8,each time).Another 20 SD rats were randomly divided into two groups:control group 2 (Sham scald group,n=10) and scald injury group 2 (n=10).Blood samples of scald injury group 2 were collected at 12 hours after scald.The rats in scald injury group 1 and 2 were placed into scalding water (95.0±0.5)℃ for 15 seconds to model third-degree burn with 30％ total burn surface area (TBSA).The rats in scald injury group 1 were given intraperitoneal injection with normal saline(40 ml/kg) immediately,while those in scald injury group 2 were given intraperitoneal injection with normal saline (40 ml/kg) 6 hours after scald.The rats in Sham scald group 1 and 2 were placed into warm water 37℃ for 15 seconds,and did not received injection.Portable glucometer/caudal artery (vein) blood,portable glucometer/abdominal aorta blood,spectrophotometer/femoral venous plasma,and spectrophotometer/abdominal aorta plasma were used to detect blood glucose.Results ①Compared with Sham scald group 1,the levels of blood glucose detected by portable glucometer/caudal artery (vein) blood and spectrophotometer/femoral venous plasma in scald injury group1 at 12,24 hours after scald were significantly increased(P＜0.05).Compared with Sham scald group 2,the levels of blood glucose detected by portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma in scald injury group 2 at 12 hours after scald were significantly increased(P＜0.05).②The comparison of portable glucometer/caudal artery (vein) blood and spectrophotometer/femoral venous plasma in Sham scald group 1,portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma in Sham scald group 2 had no statistical significance (P＞0.05).The levels of blood glucose detected by portable glucometer/caudal artery (vein) blood were significantly lower than those detected by spectrophotometer/femoral venous plasma (P＜0.05) in scald injury group 1.The comparison of blood glucose detected by portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma had no statistical significance in scald injury group 2 (P＞0.05).Conclusion Four kinds of methods used in this study shows that the levels of blood glucose were significantly increased at early stage in severely burned rats,and the portable glucometer/abdominal aorta blood is a relatively simple and fast method to detect blood glucose.
