Bone marrow mesenchymal stem cells transplantation for repair of the hippocampal neurons after cardiac arrest in rats
10.3760/cma.j.issn.1671-0282.2017.04.010
- VernacularTitle:骨髓间充质干细胞移植修复心搏骤停后大鼠海马神经元损伤
- Author:
Feng CHEN
;
Qingming LIN
;
Xiahong TANG
;
Jun KE
;
Shen ZHAO
;
Zheng GONG
;
Haiyang SONG
;
Yufeng CHEN
- Keywords:
BMSCs;
Cardiac arrest;
Cerebral resuscitation;
Hippocampal neurons;
Cerebral protection
- From:
Chinese Journal of Emergency Medicine
2017;26(4):405-409
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the improvement of ischemic hypoxic injury of brain after the transplantation of bone marrow mesenchymal stem cells (BMSCs).Methods Rats were randomly (random number) divided into sham operation group,cardiac arrest group and BMSCs treatment group (n =10 in each group).The model of cardiac arrest was induced by asphyxia.One hour after restoration of spontaneous circulation (ROSC),green fluorescent protein labeled BMSCs were transplanted via tail vein injection.At 3 and 7 days after transplantation,frozen sections of hippocampus was stained with hematoxylin-eosin (HE).The rest of brain tissue was weighed by an electronic balance.Brain water content (％) was calculated as (wet weight-dry weight) / wet weight × 100％.Results ①BMSCs were observed in hippocampus at 3 and 7 days after transplantation under fluorescent microscopy.②Compared with sham operation group and BMSCs treatment group,brain water content in cardiac arrest group was higher (all P ＜ 0.05).HE staining results showed that BMSCs transplantation could lessen hypoxia ischemia damage on brain.Conclusions BMSCs reduced the neurons damage induced by cardiac arrest and promoted neurological function recovery.
