Study on the expression level of 8-Hydroxy-2'-deoxyguanine in rhesus monkeys of different ages
10.3760/cma.j.issn.0254-9026.2017.01.022
- VernacularTitle:不同年龄恒河猴羟基脱氧乌苷表达水平的研究
- Author:
Fei SHI
;
Ben NIE
;
Wei GAN
;
Yanqiu ZHOU
;
Jianping CAI
- Keywords:
Deoxyguanosine;
Aging;
Macaca mulatta;
Free radicals
- From:
Chinese Journal of Geriatrics
2017;36(1):84-87
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the expression of 8-Hydroxy-2'-deoxyguanine(8-oxodG)in white blood cell,plasma and urine of rhesus monkey of different age group.Methods 30 female rhesus macaques at different age(1y,5y,10y,15y,20y,25y)were selected and grouped(n=5,each).10 mL of morning urine and 5 mL of fasting venous blood were collected.The level of 8-oxodG expression in plasma,leukocyte and urine was measured by high-performance liquid chromatography-mass spectrometry(HPLC-MS) method.Results The level of 8-oxodG in leukocytes,plasma and urine was increased along with aging.The level of 8-oxodG was 1.8,1.6 and 1.4 times higher in 25 year group than in 1 year group in plasma,white blood cell and urine,respectively(P＜0.05).The 8-oxodG level was more than 40 times higher in urine than in plasma.Conclusions The expression level of 8-oxodG is increased along with aging.It may be one of the experimental evidence of the aging markers.
