The relationship between colon cancer and miR-155-associated DNA methylation
10.3760/cma.j.issn.0254-9026.2017.01.018
- VernacularTitle:miR-155相关DNA甲基化与结肠癌的关系
- Author:
Peisheng HE
;
Jingxiang ZHOU
- Keywords:
Colonic neoplasms;
MicroRNAs
- From:
Chinese Journal of Geriatrics
2017;36(1):68-70
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To detect the expression level of miR-155 in tissues of colon cancer,and to investigate its influence on the occurrence,development and metastasis of colon cancer.Methods 69 cases with primary colon cancer treated with surgical resection in our hospital were chosen.Tissue samples of the tumor and tissue in the side of tumor(control)were collected and the expression level of miR-155 in the samples was detected by fluorescence quantitative polymerase chain reaction.The results were statically analyzed.Results The expression level of miR-155 was 0.471±0.100,0.238 ±0.108,0.719 ± 0.135 in three groups of colon cancer patients with tumor sizes ≥ 5 cm,high differentiation of tumor,and occurrence of lymphatic metastasis,respectively,and 0.623 ± 0.134,0.142 ±0.094,0.390±0.121 in their controls.And the level of miR-155 was 0.395±0.101 in patients with T1 + T2 tumor local infiltration,0.466±0.128 in T3 + T4 local infiltration,0.404±0.108 in Ⅰ + Ⅱ stage of TNM and 0.457±0.119 in Ⅲ± Ⅳ stage.The miR-155 was relatively higherly expressed in patients with tumor sizes≥5 cm,lower degree of differentiation,higher tumor infiltration degree,Ⅲ and Ⅳ stage of TNM,(t=3.002,12.531,20.425,2.576,1.908,P ＜ 0.05).Conclusions The expression level of miR-155 is correlated with tumor sizes,differentiation degrees,tumor infiltrating degrees,TNM and lymphatic metastasis,which could be used as diagnostic indexes for occurrence,development and metastasis of colon cancer.
