Dynamic evaluation of myocardial dysfunction in rats after cardiopulmonary resuscitation
10.3760/cma.j.issn.1671-0282.2017.01.009
- VernacularTitle:大鼠心肺复苏后心功能不全动态评价模型的研究
- Author:
Shen ZHAO
;
Yumin HE
;
Bing XU
;
Heng LI
;
Musen DAI
;
Feng CHEN
- Keywords:
Cardiac arrest;
Cardiopulmonary resuscitation;
Myocardial dysfunction;
Mitochondrion;
Animal model
- From:
Chinese Journal of Emergency Medicine
2017;26(1):44-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of myocardial structure and dysfunction during post-resuscitation period in order to establish a rat mode of post-resuscitation myocardial dysfunction after cardiac arrest resulted from electric stimulation-induced ventricular fibrillation (VF) and cardiopulmonary resuscitation (CPR).Methods A total of 40 male Sprague-Dawley (SD) rats were randomly (random number) assigned into post-resuscitation (PR) 4 h,PR 12 h,PR 24 h,PR 72 h and sham groups.VF was induced by an alternating electric current delivered to the right ventricular endocardium and untreated for 8 min.Biphasic waveform defibrillation was attempted and mechanical ventilation was synchronized after 6 min of CPR.Myocardial function was assessed with serum myocardial enzyme activity,echocardiography,mitochondrial respiratory function and histopathologic findings at different intervals.Results Thirty-two animals were successfully resuscitated with restoration of spontaneous circulation (ROSC) in 86％ (32/37) rats.Compared with sham group,severe systolic and diastolic heart failure were found at 4 h after ROSC and then gradually improved without significant difference (P ＞0.05) in ejection fraction at PR 72 h after ROSC was found,whereas thickened ventricular wall and increased myocardial performance index as well as interstitial proliferation were observed at 72 h after ROSC.Conclusions A rat model of post-resuscitation myocardial dysfunction after cardiac arrest resulted from electric stimulation-induced VF and CPR was successfully established.
