Construction of vascular sustained release model of vascular endothelial growth factor
10.3760/cma.j.issn.1001-4497.2016.10.011
- VernacularTitle:载血管内皮生长因子组织工程血管缓释模型构建
- Author:
Xiaolong MA
;
Wenbin LI
;
Zhifei XIN
;
Diankun LI
;
Zifan ZHOU
;
Juyi WAN
;
Xu MENG
- Keywords:
Tissue engineering;
Vascular endothelial growth factor;
Delayed-action preparations;
Models,animal
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2016;32(10):625-628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a model of small caliber vascular endothelial growth factor(VEGF) in tissue engineering,to investigate the performance of the sustained-release microspheres and vascular stent,and to provide materials and theoretical basis for animal experiment.Methods The sheep carotid arteries were treated with a cellular reagents,the cellular conditions and the stent properties were observed.Preparation of sustained release microspheres containing VEGF,particle size,encapsulation efficiency,drug loading and release curve were measured.The effective combination of the slow release microsphere and the vascular stent was used in the freeze drying technology.The rat vascular endothelial cells grown in tissue engineered blood vessel model release lumen,observe the growth of endothelial cells.Results After the treatment,the original performance of the vascular stent can be maintained.The average particle size of the microspheres was (9.8 ± 6.0) μm,which could be released slowly in 20 days,and the release rate was 70％.Microspheres can effectively with the tissue.engineering blood vessel tight binding.Rat vascular endothelial cells can grow in the vascular stent surface.Conclusion Using Triton X-100,DNA/RNA ribozyme for acellular reagent,stent performance is good.PLGA microspheres have good sustained release performance,and constructing appropriate tissue engineered small caliber vascular release model by using freeze drying technology can make the stent compact structure.
