Effects of differential protein transgelin-2 overexpression on biological characteristics of colon cancer cell lines
10.3760/cma.j.issn.1007-631X.2016.12.012
- VernacularTitle:差异蛋白transgelin-2过表达对结肠癌细胞生物学行为的影响
- Author:
Jing ZHOU
;
Yanbin ZHANG
;
Hui ZHANG
;
Bin LIANG
;
Yingjiang YE
;
Shan WANG
- Keywords:
Colonic neoplasms;
Neoplasm invasiveness;
Transgelin-2
- From:
Chinese Journal of General Surgery
2016;31(12):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of transgelin-2 expression on biological characteristics of colon cancer cells.Methods RT-PCR and Western blot were used to observe mRNA and protein expression of transgelin-2 in five colon cancer cell lines,screen for cell line with lower transgetin-2 expression;Transient transfection was performed to establish over-expression of transgelin-2 colon cancer cell line;The effect of transgelin-2 over-expression on the proliferation,apoptosis and the ability of migration and invasion of colon cancer cell were detected by CCK-8,low cytometric analysis and transwell method respectively.Results There was no statistical difference in proliferation and apoptosis in colon cancer cells with transgelin-2 over-expression compared with the controls;Enhanced ability of migration and invasion was found in colon cancer cells with transgelin-2 over-expression.After 15 hours culture in serum free medium,more transgelin-2 over-expressed colon cancer cells went through the transwell chamber bottom mnembrane than that in control group and empty vector transfected group 207 ±62 vs.114 ±29 vs.120 ±26,F =7.302,P ＜0.05).After 24 hours,the differences remained statistically significant (179 ± 32 vs.95 ± 33 vs.95 ± 28,F =10.960,P ＜ 0.05).Conclusions Transgelin-2 enhances migration and invasion ability of colon cancer cells.
