Rat perfusion-acellular pancreatic bioscaffold as a natural 3D platform
10.3760/cma.j.issn.1007-631X.2016.12.014
- VernacularTitle:灌注法制备大鼠全胰腺脱细胞基质支架的实验研究
- Author:
Xin WANG
;
Hongbin CHENG
;
Yuan LI
;
Yue DU
- Keywords:
Pancreas;
Regenerative medicine;
Acellular pancreatic bioscaffold;
Perfusion
- From:
Chinese Journal of General Surgery
2016;31(12):1034-1037
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biochemical properties of rat improved perfusion-acellular pancreatic bioscaffold (APB).Methods The fresh pancreas from 10 rats were perfused through portal vein.The histological structure,ECM composition and DNA quantification of APB were evaluated.For the biocompatibility study,a 0.5 cm2 APB construct was surgically placed within a dorsal subcutaneous pocket of mice.Results The pancreatic tissue become translucent and the macroscopic three-dimensional architectures of native pancreas are retained after decellularization.The extracellular matrix (ECM) ultrastructures were well preserved.Immunofluorescence staining showed that collagen Ⅰ,Ⅳ,fibronectin and laminin were maintained in the APB after decellularization.DNA quantification of APB decreased significantly (P ＜ 0.05).The histological analysis of the subcutaneous implantation site showed the presence of immunological response surrounding the partially degraded APB.The histological remold score was 10.4 ± 1.8 at 14 days and 13.8 ± 1.3 at 28 days.The proliferation of AR42J cell line with the expression of amylase as the marker of exocrine acinar cells can be detected on the recellularized APB.Conclusion APB in this study met the stringent requirement to define a successful decellularization.The technique allowed the efficient generation of APB with preserved 3D architecture and represented a biocompatible scaffold capable of integrating within host tissue.
