A study of radiation injury in rat C6 glioma cell line by 1H-nuclear magnetic resonance spectroscopy
10.3760/cma.j.issn.1004-4221.2017.02.021
- VernacularTitle:应用MR氢谱检测大鼠脑胶质瘤C6细胞放射损伤
- Author:
Hongxia LI
;
Yi CUI
;
Yanjie XU
;
Fuyan LI
;
Wenqi SHI
;
Jianzhen WANG
;
Qingshi ZENG
- Keywords:
Deoxyribonucleic acid damage;
Proton magnetic resonance;
Cell line,glioma;
Apoptosis;
Cell cycle arrest
- From:
Chinese Journal of Radiation Oncology
2017;26(2):228-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiation injury of rat C6 glioma cell line by high resolution,1 H-nuclear magnetic resonance (1 H NMR) spectroscopy,and to preliminarily investigate its mechanism.Methods Metabolite concentrations in C6 cells were determined by 1 H NMR spectroscopy.Comet assay was used to evaluate DNA damage.Flow cytometry was used to determine the cell cycle and apoptosis rate.Colony-forming assay was used to measure the colony-forming rate and preliminarily investigate the mechanism of radiation injury.The resuhs were analyzed by one-way analysis of variance and Pearson correlation analysis.Results With the increase in radiation dose from 0 Gy to 1,5,10,and 15 Gy,DNA damage was enhanced in a dose-dependent manner (P=0.000-0.690);the percentage of cells in G1 phase increased (P =0.026-0.749);the apoptosis rate significantly increased (all P =0.000);the colony-forming rate significantly declined (P =0.000-0.004);the Lac/Cr ratio significantly decreased (P =0.000-0.015),which had a negative linear correlation with DNA damage parameters (tail length,r=-0.971;％DNA in the tail,r =-0.998;tail moment,r =-0.995) and apoptosis rate (r =0.978).Conclusions 1 H NMR spectroscopy reveals that the change in the Lac/Cr ratio is associated with injury and apoptosis of C6 cells after radiation.1 H NMR spectroscopy has the potential to predict radiation injury of glioma.
