Expression Profile of Fas-Fas Ligand in Spiral Ganglion Cells During Apoptosis.
- Author:
Tae Woo GU
1
;
Woo Yong BAE
;
Hwan Tae PARK
;
Jae Hoon LEE
;
Min Young KANG
;
Sung Wook JEONG
;
Yoon Kyung SHIN
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea. doncamel@dau.ac.kr
- Publication Type:Original Article
- Keywords:
Spiral ganglion cell;
Glutamate;
Apoptosis;
Fas ligand;
Caspase 3
- MeSH:
Apoptosis*;
Caspase 3;
Fas Ligand Protein;
Fluorescent Antibody Technique;
Glutamic Acid;
Spiral Ganglion*
- From:Clinical and Experimental Otorhinolaryngology
2014;7(1):1-6
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis. METHODS: Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining. RESULTS: In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner. CONCLUSION: FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.
