Decreased Pattern-Recognition Receptor-Mediated Cytokine mRNA Expression in Obese Children With Otitis Media With Effusion.
- Author:
Youn Jung KIM
1
;
Sung Ho CHA
;
Ho Yun LEE
;
Sun Kyu LEE
;
Hee Yong CHUNG
;
Joon Hyung YEO
;
Young Il KIM
;
Seung Geun YEO
Author Information
1. Kyung Hee University College of Nursing Science, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Otitis media;
Obesity;
Pattern-recognition receptors;
Immunoglobulin;
Immunity;
Bacteria
- MeSH:
Bacteria;
Body Mass Index;
Child*;
Humans;
Immunity, Humoral;
Immunoglobulin A;
Immunoglobulin G;
Immunoglobulin M;
Immunoglobulins;
Interferons;
Interleukin-10;
Interleukin-12;
Interleukin-6;
Interleukins;
Obesity;
Otitis Media with Effusion*;
Otitis Media*;
Otitis*;
RNA, Messenger*;
Toll-Like Receptors;
Tumor Necrosis Factor-alpha
- From:Clinical and Experimental Otorhinolaryngology
2014;7(1):7-12
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: To assess innate and humoral immune responses in middle ear effusion of obese pediatric patients with otitis media with effusion (OME). METHODS: We evaluated 219 children with OME, of whom 21 were obese and 198 were non-obese. We compared the expression in middle ear effusion of mRNAs encoding toll-like receptors (TLR) 2, 4, 5, and 9; nucleotide-binding oligomerization domains (NOD) 1 and 2; retinoic acid-inducible gene (RIG)-I; interleukins (IL)-6, -10, and -12; interferon (IFN)-gamma; and tumor necrosis factor (TNF)-alpha mRNAs. We also compared the expression of immunoglobulins IgG, IgA, and IgM and the bacterial detection rate in the two groups. RESULTS: TLR2-mediated expression of IL-6 mRNA, TLR4-mediated expression of IL-6 and IL-10 mRNA, TLR5-mediated expression of IL-6, IL-10, and TNF-alpha mRNA, TLR9-mediated expression of IL-6 mRNA, and NOD2-mediated expression of IL-6, IL-12, and TNF-alpha mRNA were significantly lower in obese than in non-obese children (P<0.05). However, concentrations of IgG, IgA, and IgM in middle ear effusion were lower in obese than in non-obese children, but none of these differences was significant (P>0.05). CONCLUSION: Mean body mass index was higher and pattern-recognition receptor-mediated cytokine mRNA expression was lower in obese than in non-obese children with OME.
