Effects of Alprostadil on the Expression of VEGF and eNOS in Human Pulmonary Microvascular Endothe-lial Cells with Hypoxia-induced Injury
10.6039/j.issn.1001-0408.2016.25.19
- VernacularTitle:前列地尔对缺氧损伤的人肺微血管内皮细胞中VEGF和eNOS表达的影响
- Author:
Youjun WANG
;
Lingmei ZHANG
- Publication Type:Journal Article
- Keywords:
Alprostadil;
Hypoxia-induced injury;
Human pulmonary microvascular endothelial cells;
Vascular endothelial growth factor;
Endothelial nitric oxide synthase
- From:
China Pharmacy
2016;27(25):3518-3520
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of alprostadil on the expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in human pulmonary microvascular endothelial cells (HPMECs) with hypoxia-in-duced injury. METHODS:HPMECs were divided into group A(normal cultural environment),group B(3%O2),group C(3%O2+15 μg/L alprostadil)and group D(3%O2+45 μg/L alprostadil)for 24 h culture. Cell morphology was observed;MTT method was conducted to detect cell activity(recorded by absorbance);flow cytometry was adopted to detect apoptosis rate;Western blot was used to detect relative expressions of VEGF and eNOS in cells. RESULTS:Cell density in group A and D was relatively high, that in group B and C was low. Compared with group A,cell activities decreased and apoptosis rates increased in group B,C and D,expressions of VEGF and eNOS enhanced,eNOS/VEGF decreased (P<0.05). Compared with group B,cell activities in-creased in group C and D,VEGF expression decreased (P<0.05);apoptosis rate in group D decreased,eNOS expression de-creased and eNOS/VEGF increased. Compared with group C,cell apoptosis rate was decreased in group D,VEGF expression de-creased and eNOS/VEGF increased(P<0.05). CONCLUSIONS:Alprostadil maybe play effect on HPMECs with hypoxia-induced injury by up-regulating the expression level of eNOS/VEGF.
