Study on the Quality Standard of Flemingia philippinensis
10.6039/j.issn.1001-0408.2016.24.27
- VernacularTitle:千斤拔药材的质量标准研究
- Author:
Jinfeng LIN
;
Qingming LI
;
Yufang ZHU
;
Haoan CHEN
- Publication Type:Journal Article
- Keywords:
Flemingia philippinessis;
Flemingia macrophylla;
Quality standard;
TLC;
HPLC;
Genistin;
Genistein
- From:
China Pharmacy
2016;27(24):3400-3403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard of Flemingia philippinensis. METHODS:The properties and micro-scopic characteristics were observed;TLC was adopted for qualitative identification of genistin and genistein;the moisture,total ash and extract were detected;HPLC was adopted for contents determination of genistin and genistein:the column was Thermo BE-TASIL C18 with mobile phase of Acetonitrile-0.5% glacial acetic acid(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 261 nm,column temperature was 25℃,and the injection volume was 10μl. RESULTS:The properties and micro-scopic characteristics of F. philippinensis and F. macrophylla showed strong specificity. TLC spots of genistin and genistein we- re clear and well separated,with no interference in negative control. The moisture was 3.69%-8.37%,total ash was 1.72%-6.74%,and extract was 5.89%-19.65%. The linear range was 0.012 9-2.588 μg for genistin(r=0.999 9)and 0.004 6-0.923 2 μg for genistein (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 99.63%-101.87%(RSD=0.82%,n=6)and 97.19%-100.34%(RSD=1.23%,n=6). CONCLUSIONS:The method is simple,accurate and specif-ic,and can be used for the quality control of F. philippinensis.
