Simultaneous Determination of Four Components in Weisu Granule by UPLC
10.6039/j.issn.1001-0408.2016.24.41
- VernacularTitle:UPLC法同时测定胃苏颗粒中4种成分的含量
- Author:
Yinrui GUO
;
Guangchang ZHANG
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
UPLC;
Weisu granule;
Narirutin;
Hesperiden;
Neohesperidin;
Naringin;
Content determination
- From:
China Pharmacy
2016;27(24):3443-3445
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of narirutin,naringin,hesperiden and neo-hesperiden in Weisu granule. METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.2% Phosphoric acid aqueous(gradient elution)at a flow rate of 0.40 ml/min,the detection wavelength was 284 nm, the column temperature was 30 ℃,and the injection volume was 1 μl. RESULTS:The linear range was 9.38-93.75 μg/ml for narirutin(r=0.999 7), 32.25-322.50 μg/ml for naringin(r=0.999 7), 11.25-112.50 μg/ml for hesperiden(r=0.999 9) and 11.88-118.75 μg/ml for neohesperidin(r=0.999 8);limits of quantitation were 20 ng,18 ng,18 ng and 18 ng,the limits of detec-tion were 6 ng,5 ng ,5 ng and 5 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;re-coveries were 96.24%-103.12%(RSD=2.45%,n=6),98.43%-102.10%(RSD=1.42%,n=6),96.10%-101.41%(RSD=2.07%,n=6)and 95.57%-99.06%(RSD=1.44%,n=6),respectively. CONCLUSIONS:The method is rapid and efficient,and suitable for the simultaneous determination of narirutin,naringin,hesperiden and neohesperiden in Weisu granule.
