Simultaneous Determination of Chlorogenic Acid,Evodiamine and Rutecarpine in Different Places of Evodia rutaecarpa by UPLC
10.6039/j.issn.1001-0408.2016.24.42
- VernacularTitle:UPLC法同时测定不同产地吴茱萸中绿原酸、吴茱萸碱、吴茱萸次碱的含量
- Author:
Weimiao WU
;
Jinying LUO
- Publication Type:Journal Article
- Keywords:
UPLC;
Evodia rutaecarpa;
Chlorogenic acid;
Evodiamine;
Rtecarpine;
Content determination
- From:
China Pharmacy
2016;27(24):3446-3448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,evodiamine,rutecar-pine in different places of Evodia rutaecarpa. METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.1% Phosphoric acid aqueous solution(gradient elution)at a flow rate of 0.40 ml/min,the detection wavelength was 326 nm and 220 nm,column temperature was 30 ℃,and the volume injection was 2 μl. RESULTS:The linear range was 7.67-76.67 μg/ml for chlorogenic acid(r=0.999 2),13.33-133.33 μg/ml for evodiamine(r=0.999 7)and 13.33-133.33μg/ml for rutecarpine(r=0.999 8);the limits of quantitation were 0.11 ng,0.05 ng and 0.05 ng,the limits of detection were 0.03 ng,0.01 ng and 0.01 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 96.19%-101.90%(RSD=2.19%,n=6),95.35%-101.16%(RSD=2.27%,n=6)and 95.92%-98.98%(RSD=1.33%,n=6),re-spectively. CONCLUSIONS:The method is rapid and accurate,and suitable for the simultaneous determination of chlorogenic ac-id,evodiamine,rutecarpine in different places of E. rutaecarpa.
